|Reactivity||Human, Mouse, Rat|
|Calculated MW||46426 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Other applications have not been determined. The antibody detects ~46 kDa Smad-7 from samples of human, mouse, and rat origins. Reactivity to other species has not been determined.|
|Other Names||SMAD7 , MADH7, MADH8 , Smad7; hSMAD7; SMAD family member 7|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Smad7 polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Smad7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A- PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.|
|Cellular Location||Nucleus Cytoplasm Note=Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236)|
|Tissue Location||Ubiquitous with higher expression in the lung and vascular endothelium|
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Provided below are standard protocols that you may find useful for product applications.
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1, Smad5, and Smad8 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF-β and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities thro µgh interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.
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