|Application ||WB, IP|
|Calculated MW||71277 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). Jurkat cell lysate and rat liver or rat kidney tissue lysate can be used as a positive control. A ~75 kDa band should be detected.|
|Other Names||FOXO3A , 602681 , O43524 , AF6q21 , DKFZp781A0677 , FKHRL1 , FKHRL1P2 , MGC12739 , MGC31925|
|Formulation||100 µg (0.2mg/ml) affinity purified rabbit anti-FOXO3a polyclonal antibody in phosphate-buffered saline (PBS) containing 0.5% BSA, 30% glycerol, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||FOXO3a Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post- transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.|
|Cellular Location||Cytoplasm, cytosol. Nucleus. Note=Translocates to the nucleus upon oxidative stress and in the absence of survival factors|
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Provided below are standard protocols that you may find useful for product applications.
Mammalian forkhead members of the class O (FOXO) transcription factors, including FOXO1, FOXO3a, and FOXO4, are implicated in the regulation of a variety of cellular processes, including the cell cycle, apoptosis, DNA repair, stress resistance, and metabolism. FOXO proteins are negatively regulated by the phosphatidylinositol 3-kinase-Akt signaling pathway, which is activated by growth factors and cytokines. Recent studies indicate that the activities of FOXO proteins are also regulated by oxidative stress, which induces their phosphorylation, translocation to the nucleus, and acetylation-deacetylation. Similar to the tumor suppressor p53, FOXO is activated by stress and induces the expression of genes that contribute to cell-cycle arrest, s µggesting that it also functions as a tumor suppressor.
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