|Application ||WB, E|
|Reactivity||Human, Bovine, Horse|
|Clone Names||biG 10|
|Calculated MW||40076 Da|
|Application & Usage||Suitable for immunostaining of CD14positive cells (Flow Cytometry); ELISA; CD14 inhibition studies; Western Blot|
|Other Names||CD14, Cluster of differentiation 14|
|Formulation||Human CD-14 was lyophilized from a concentrated protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||CD-14 Antibody (Clone biG 10) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor.|
|Tissue Location||Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages|
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Provided below are standard protocols that you may find useful for product applications.
The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.
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