|Reactivity||Human, Mouse, Rat|
|Calculated MW||62310 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Other applications have not been tested.|
|Other Names||PAK3 , PAK3beta , Beta-PAK , hPAK3 , MRX47 , MRX30 , bPAK , oligophrenin-3 , CDKN1A , OPHN3|
|Formulation||100 µg (0.5 mg/ml) Protein A affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||PAK3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development.|
|Tissue Location||Restricted to the nervous system. Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus.|
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Provided below are standard protocols that you may find useful for product applications.
The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal recognization, MAPK signaling, apoptotic signaling, control of phagocytes, NADPH oxidase and growth factor-induced neurite outgrowth. Research indicates that phosphorylation of serine 139 of PAK3 which is located in the kinase inhibitory domain, affects the kinase activity.
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