PAK3 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | O75914 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 62310 Da |
Gene ID | 5063 |
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Application & Usage | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Other applications have not been tested. |
Other Names | PAK3 , PAK3beta , Beta-PAK , hPAK3 , MRX47 , MRX30 , bPAK , oligophrenin-3 , CDKN1A , OPHN3 |
Target/Specificity | PAK3 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml) Protein A affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | PAK3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PAK3 |
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Synonyms | OPHN3 |
Function | Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, or cell cycle regulation. Plays a role in dendrite spine morphogenesis as well as synapse formation and plasticity. Acts as a downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration. Additionally, phosphorylates TNNI3/troponin I to modulate calcium sensitivity and relaxation kinetics of thin myofilaments. May also be involved in early neuronal development. In hippocampal neurons, necessary for the formation of dendritic spines and excitatory synapses; this function is dependent on kinase activity and may be exerted by the regulation of actomyosin contractility through the phosphorylation of myosin II regulatory light chain (MLC) (By similarity). |
Cellular Location | Cytoplasm. |
Tissue Location | Restricted to the nervous system. Highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The p21-activated kinase (PAK) family of serine/threonine kinases is engaged in multiple cellular processes, including cytoskeletal recognization, MAPK signaling, apoptotic signaling, control of phagocytes, NADPH oxidase and growth factor-induced neurite outgrowth. Research indicates that phosphorylation of serine 139 of PAK3 which is located in the kinase inhibitory domain, affects the kinase activity.
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