|Calculated MW||70642 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~65 kDa HAT-2 from human samples, and in a lesser extent, from mouse and rat samples. Jurkat cell lysate can be used as a positive control.|
|Other Names||Anti-HAT-2 antibody, HAT-2 antibody, Anti-HAT-2, HAT-2, HAT, histone acetyltransferase, HATs, HDACs HAT-2 Polyclonal Antibody, Anti-HAT-2|
|Formulation||100 µg (0.5 mg/ml) Protein A affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||HAT-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||HBO1, HBOa, MYST2|
|Function||Component of the HBO1 complex which has a histone H4- specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription.|
|Cellular Location||Nucleus, nucleoplasm|
|Tissue Location||Ubiquitously expressed, with highest levels in testis.|
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Provided below are standard protocols that you may find useful for product applications.
Histone acetyltransferases (HATs) have been implicated in a number of cellular functions including gene regulation, DNA synthesis, and repair. Histone acetyltransferases and deacetylases are, respectively, the enzymes devoted to the addition and removal of acetyl groups from lysine residues on the histone N-terminal tails. The enzymes exert fundamental roles in developmental processes and their deregulation has been linked to the progression of diverse human disorders, including cancer.
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