|Calculated MW||21879 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Rat kidney tissue lysate can be used as a positive control. The antibody recognizes ~24 kDa NCS-1 from samples of human and rat origins. Reactivity to other species has not been determined.|
|Other Names||FREQ , FLUP , NCS1 , ANTI NEURONAL CALCIUM SENSOR 1 , Frequenin , NCS-1|
|Formulation||100 µg (0.5 mg/ml) Protein A affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||NCS-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin (By similarity). Stimulates PI4KB kinase activity (By similarity). Involved in long-term synaptic plasticity through its interaction with PICK1 (By similarity). May also play a role in neuron differentiation through inhibition of the activity of N- type voltage-gated calcium channel (By similarity).|
|Cellular Location||Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cytoplasm, perinuclear region. Cell membrane; Peripheral membrane protein Note=Associated with Golgi stacks. Post-synaptic densities of dendrites, and in the pre-synaptic nerve terminal at neuromuscular junctions|
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Provided below are standard protocols that you may find useful for product applications.
The neuronal calcium sensor (NCS) protein binds to calcium thro µgh EF-hand motifs, are expressed in neurons. They are implicated in the regulation of neurotransmitters released at the neuromuscular junctions. NCS-1 is widely distributed in the brain, spinal cord and dorsal root ganglia. It has been shown to regulate neurosecreation in a calcium-dependent manner, potentiates nitric oxide synthase activity. NCS-1 has been reserved to indicate the protein observed in humans and mammals.
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