|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||40796 Da|
|Application & Usage||Western blotting (2-6 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 40 kDa BIF-1 in samples from human, mouse, and rat origins. Jurkat cell lysate and rat kidney tissue lysates can be used as positive controls.|
|Other Names||Bax-interacting factor 1 , Bif-1 , CGI-61 , Endophilin 3 , Endophilin B1 , KIAA0491 , SH3GLB1 , SH3p13|
|Formulation||100 µg (0.2 mg/ml) Protein A affinity purified rabbit anti-BIF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||BIF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion.|
|Cellular Location||Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein. Note=Association with the Golgi apparatus depends on the cell type.|
|Tissue Location||Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes.|
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Provided below are standard protocols that you may find useful for product applications.
BIF-1 (Bax interacting factor-1) is a 365 amino acid protein that interacts with Bax protein and influences cell survival and death. BIF-1 contains a Src homology 3 (SH3) domain in the C-terminus. Overexpression of BIF-1 promotes Bax conformational change, caspase activation, and apoptotic cell death.
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