|Calculated MW||225028 Da|
|Application & Usage||Western blotting (2-6 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody recognizes 220 kDa human HAT-3. A 50 kDa unknown band can also be detected. Reactivity to other species has not been determined.|
|Other Names||Histone acetyltransferase , MYST , RUNXBP2 , SAS2 , TIP60 , MOZ|
|Formulation||100 µg (0.5 mg/ml) Protein A affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||HAT-3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||MOZ, MYST3, RUNXBP2, ZNF220|
|Function||Histone acetyltransferase that acetylates lysine residues in histone H3 and histone H4 (in vitro). Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. May act as a transcriptional coactivator for RUNX1 and RUNX2. Acetylates p53/TP53 at 'Lys-120' and 'Lys-382' and controls its transcriptional activity via association with PML.|
|Cellular Location||Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus, PML body. Note=Recruited into PML body after DNA damage|
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Provided below are standard protocols that you may find useful for product applications.
Histone acetyltransferases (HATs) have been implicated in a number of cellular functions including gene regulation, DNA synthesis, and repair. Histone acetyltransferases and deacetylases are respectively, the enzymes devoted to thr addition and removal of acetyl groups from lysine residues on the Histone N-terminal trails. The enzymes exert fundamental roles in developmental processes and their deregulation has been linked to the progression of diverse human disorders, including cancer.
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