|Application ||WB, IHC|
|Calculated MW||147870 Da|
|Positive Control||Western Blot: Jurkat cell lysate . IHC: Brain and Cortex tissue|
|Application & Usage||Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 139 kDa human PLCg2. Jurkat cell lysate can be used as a positive control. Reactivity to other species has not been determined.|
|Other Names||PLC2 , PLC148 , PLC-II , PLC-148, inositoltrisphosphohydrolase|
|Target/Specificity||PLC gamma 2|
|Formulation||100 µg (0.5 mg/ml) protein A affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||PLC gamma 2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.|
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Provided below are standard protocols that you may find useful for product applications.
PLC (Phosphoinositide-specific phospholipase C) plays a significant role in transmembrane signaling. Four members of PLCs have been identified: PLCβ, PLCg, PLCd, and PLCe. In response to extracellular stimuli (e.g., hormone, growth factors, neurotransmitters), PLC hydrolizes phosphatidylinositol 4,5-biphosphate (PIP2) into two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PLCg2 is engaged in antigen-dependent signaling in B-cells and collagen-dependent signaling in platelets.
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