BRD8 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
---|---|
Primary Accession | Q9H0E9 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 135336 Da |
Gene ID | 10902 |
---|---|
Application & Usage | Western blotting (1-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects BRD8 in Human, mouse and rat samples. |
Other Names | BRD-8, Bromodomain containing 8, SMAP, Skeletal Muscle Abundant Protein, Thyroid hormone receptor coactivating protein p120 |
Target/Specificity | BRD8 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA, and 0.02% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | BRD8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | BRD8 |
---|---|
Synonyms | SMAP, SMAP2 |
Function | May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome. |
Cellular Location | Nucleus. |
Tissue Location | Expressed in adipose tissue, brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
BRD8 interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. BRD8 contains a bromodomain and is tho µght to be a nuclear receptor coactivator. Three alternatively spliced BRD8 transcript variants that encode distinct BRD8 isoforms have been identified.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.