Cathepsin L (Cleaved) Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC |
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Primary Accession | P07154 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 37660 Da |
Gene ID | 25697 |
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Application & Usage | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes the mature form of Cathepsin L (25 kDa) from human, mouse and rat origins. Reactivity to other species has not been tested. Rat kidney tissue lysate can be used as a positive control. |
Other Names | CTSL1 , CATL , CTSL , MEP , FLJ31037 , EC 3.4.22.15 , OTTHUMP00000063566 |
Target/Specificity | Cathepsin L (Cleaved) |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA, and 0.02% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Cathepsin L (Cleaved) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Ctsl {ECO:0000312|RGD:2448} |
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Synonyms | Ctsl1 |
Function | Thiol protease important for the overall degradation of proteins in lysosomes (By similarity). Plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone remodeling. Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen (By similarity). In neuroendocrine chromaffin cells secretory vesicles, catalyzes the prohormone proenkephalin processing to the active enkephalin peptide neurotransmitter (By similarity). In thymus, regulates CD4(+) T cell positive selection by generating the major histocompatibility complex class II (MHCII) bound peptide ligands presented by cortical thymic epithelial cells. Also mediates invariant chain processing in cortical thymic epithelial cells. Major elastin-degrading enzyme at neutral pH. Accumulates as a mature and active enzyme in the extracellular space of antigen presenting cells (APCs) to regulate degradation of the extracellular matrix in the course of inflammation (By similarity). Secreted form generates endostatin from COL18A1 (By similarity). Critical for cardiac morphology and function. Plays an important role in hair follicle morphogenesis and cycling, as well as epidermal differentiation (By similarity). Required for maximal stimulation of steroidogenesis by TIMP1 (PubMed:7777858). |
Cellular Location | Lysosome. Apical cell membrane {ECO:0000250|UniProtKB:P06797}; Peripheral membrane protein {ECO:0000250|UniProtKB:P06797}; Extracellular side {ECO:0000250|UniProtKB:P06797}. Cytoplasmic vesicle, secretory vesicle, chromaffin granule {ECO:0000250|UniProtKB:P25975}. Secreted, extracellular space {ECO:0000250|UniProtKB:P06797}. Secreted. Note=Localizes to the apical membrane of thyroid epithelial cells. Released at extracellular space by activated dendritic cells and macrophages. {ECO:0000250|UniProtKB:P06797} |
Tissue Location | Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The cysteine proteases termed lysosomal cathepsins have long been tho µght to be primarily involved in end-stage protein breakdown within lysosomal compartments. Cathepsin L functions to regulate cell cycle progression thro µgh proteolytic processing of the CDP/Cux transcription factor.
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