TPX2 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IP |
---|---|
Primary Accession | Q9ULW0 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 85653 Da |
Gene ID | 22974 |
---|---|
Application & Usage | Western blotting (1:500 – 1:2000) and Immunoprecipitation. However, the optimal concentrations should be determined individually. The antibody recognizes the human TPX2. Reactivity to other species has not been tested. |
Other Names | TPX2, TPX-2, Microtubule-associated protein TPX2 homolog, DIL2, DIL-2, Differentially expressed in lung cells, HCA519, Hepatocellular carcinoma-associated antigen 519, REPP86, Restricted expression proliferatio |
Target/Specificity | TPX2 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µl purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | TPX2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | TPX2 |
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Synonyms | C20orf1, C20orf2, DIL2, HCA519 |
Function | Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules (PubMed:18663142, PubMed:19208764, PubMed:37728657). Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation (PubMed:18663142, PubMed:19208764). TPX2 is inactivated upon binding to importin-alpha (PubMed:26165940). At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation (PubMed:26165940). |
Cellular Location | Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules. |
Tissue Location | Expressed in lung carcinoma cell lines but not in normal lung tissues |
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Provided below are standard protocols that you may find useful for product applications.
Background
TPX2 (targeting protein for Xklp2) is a microtubule-associated protein involved in targeting the motor protein Xklp2 to microtubules. Ran-GTP activates TPX2 for the chromatin-induced microbutule assembly during M phase. Aurora-A kinase associates with TPX2 at the spindle apparatus and may regulate TPX2 via phosphorylation during the spindle assembly. TPX2 appears to play a structural role in spindle formation. TPX2 activates Eg2 in a microtubule-dependent manner by stimulating the phosphorylation and kinase activity of Eg2. TPX2 is inactivated by binding to importin a, a nuclear import factor. Finally, the suppression of TPX2 with RNA interference causes defects in microtubule organization during mitosis.
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