|Calculated MW||136394 Da|
|Application & Usage||Western blotting (1:500 – 1:2000). HeLa cell nuclear extract can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the MCAF of human and mouse origins. Reactivity to other species has not been tested. |
|Other Names||MCAF, MBD1-containing chromatin associated factor, ATF7IP, activating transcription factor 7 interacting protein, hAM, human ATFa-associated Modulator, p621, HGNC:20092, FLJ10139, FLJ10688|
|Formulation||100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||MCAF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3). The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3). Facilitates telomerase TERT and TERC gene expression by SP1 in cancer cells.|
|Tissue Location||Detected at low levels in breast, lung and stomach; highly up-regulated in the corresponding cancerous tissues (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
MBD1-Containing Chromatin-Associated Factor (MCAF) associates with methyl CpG-binding protein (MBD1) that functions to recruit methyltransferases such as SETDB1 to chromatin. MCAF is required for the transcriptional repression and heterochromatin formation mediated by MBD1 and SETDB1.
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