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>   home   >   Products   >   Primary Antibodies   >   MCAF Antibody   

MCAF Antibody

Rabbit Polyclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q6VMQ6
Other Accession AAQ92978.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 136394 Da
Additional Information
Gene ID 55729
Application & Usage Western blotting (1:500 – 1:2000). HeLa cell nuclear extract can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the MCAF of human and mouse origins. Reactivity to other species has not been tested.

Other Names MCAF, MBD1-containing chromatin associated factor, ATF7IP, activating transcription factor 7 interacting protein, hAM, human ATFa-associated Modulator, p621, HGNC:20092, FLJ10139, FLJ10688
Target/Specificity MCAF
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsMCAF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MCAF, MCAF1
Function Recruiter that couples transcriptional factors to general transcription apparatus and thereby modulates transcription regulation and chromatin formation. Can both act as an activator or a repressor depending on the context. Mediates MBD1-dependent transcriptional repression, probably by recruiting complexes containing SETDB1. Required to stimulate histone methyltransferase activity of SETDB1 and facilitate the conversion of dimethylated to trimethylated H3 'Lys-9' (H3K9me3). The complex formed with MBD1 and SETDB1 represses transcription and couples DNA methylation and histone H3 'Lys-9' trimethylation (H3K9me3). Facilitates telomerase TERT and TERC gene expression by SP1 in cancer cells.
Cellular Location Nucleus.
Tissue Location Detected at low levels in breast, lung and stomach; highly up-regulated in the corresponding cancerous tissues (at protein level).
Citations (0)

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MBD1-Containing Chromatin-Associated Factor (MCAF) associates with methyl CpG-binding protein (MBD1) that functions to recruit methyltransferases such as SETDB1 to chromatin. MCAF is required for the transcriptional repression and heterochromatin formation mediated by MBD1 and SETDB1.

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$ 245.00
Cat# ABV10578
(40 western blots)
Availability: 5-7days
Bulk Size
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