|Application ||WB, IP|
|Calculated MW||68723 Da|
|Application & Usage||Western blotting (1:500 – 1:2500) and Immunoprecipitation. 293T cell lysate can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the ASH2 of human origin. Reactivity to other species has not been tested.|
|Other Names||ASH2, ASH2L1, ASH2L2, Absent, Small, or Homeotic-Like 2; Trithorax protein ASH|
|Formulation||100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||ASH2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis.|
|Tissue Location||Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes|
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Provided below are standard protocols that you may find useful for product applications.
ASH2 (absent, small, or homeotic discs 2) is a subunit of a human Set1-like histone methyltransferase (HMT) complex. The Set-1 like complexes are responsible for mono-, di-, and tri-methylation of histone H3 K4. ASH2 associates with a multitude of proteins in a number of Set1-like complexes which include various combinations of MLL, MLL2, MLL3, MLL4, RBBP5,WDR5, hDPY-30, hSwd2, CXXC1, HCF1, Menin, PTIP, PA1, NCOA6, and UTX.
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