|Application ||WB, IP|
|Calculated MW||155293 Da|
|Application & Usage||Western blotting (1:500 – 1:2500) and Immunoprecipitation. However, the optimal concentrations should be determined individually. HeLa cell lysate can be used as a positive control. The antibody recognizes the SRC3 of human origin. Reactivity to other species has not been tested.|
|Other Names||Steroid receptor coactivator 3, NCOA3, Nuclear Receptor Coactivator 3; TRAM1, Thyroid Hormone Receptor Activator Molecule 1; RAC3, Receptor-Associated Coactivator 3; AIB1, Amplified in Breast Cancer-1; pCIP, CBP-Interactin protein|
|Formulation||100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||SRC3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||AIB1, BHLHE42, RAC3, TRAM1|
|Function||Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.|
|Cellular Location||Cytoplasm. Nucleus. Note=Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus|
|Tissue Location||Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney|
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Steroid receptor coactivator 3 (SRC3) belongs to the SRC family of proteins that interacts with nuclear receptors to facilitate transcription. The 3 homologous members of this family are SRC-1 (NCoA-1), SRC-2 (GRIP1, TIF2, or NcoA-2), and SRC-3 (p/CIP, RAC3, ACTR, AIB1, or TRAM-1). SRC3 is an oncogene that is reported to be amplified and/or overexpressed in breast and gastric cancer and stimulates AKT signaling and cell growth and tumorigenesis.
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