TIF1a/TRIM24 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
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Primary Accession | O15164 |
Other Accession | NP_056989.2 |
Reactivity | Human, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 116831 Da |
Gene ID | 8805 |
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Application & Usage | Western blotting (1:500 – 1:2000). However, the optimal concentrations should be determined individually. HeLa cell lysate can be used as a positive control. The antibody recognizes the TIF1α/TRIM24 of human and mouse origins. Reactivity to other species has not been tested. |
Other Names | TF1A, TIF1, hTIF1, TIF1A, TIF1 Alpha, Transcriptional Intermediary Factor 1 Alpha, TRIM24, Tripartite Motif-Containing 24, RNF82, Ring Finger Protein 82, PTC6 |
Target/Specificity | TRIM24/TIF1a |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | TIF1a/TRIM24 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | TRIM24 |
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Synonyms | RNF82, TIF1, TIF1A |
Function | Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. During the DNA damage response, participates in an autoregulatory feedback loop with TP53. Early in response to DNA damage, ATM kinase phosphorylates TRIM24 leading to its ubiquitination and degradation. After sufficient DNA repair has occurred, TP53 activates TRIM24 transcription, ultimately leading to TRIM24-mediated TP53 ubiquitination and degradation (PubMed:24820418). Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up- regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes (By similarity). Participates also in innate immunity by mediating the specific 'Lys-63'-linked ubiquitination of TRAF3 leading to activation of downstream signal transduction of the type I IFN pathway (PubMed:32324863). Additionally, negatively regulates NLRP3/CASP1/IL- 1beta-mediated pyroptosis and cell migration probably by ubiquitinating NLRP3 (PubMed:33724611). |
Cellular Location | Nucleus. Cytoplasm. Mitochondrion. Note=Colocalizes with sites of active transcription. Predominantly nuclear. Translocated from nucleus to mitochondria to mediate antiviral immunity (PubMed:32324863). Localizes to sites of DNA damage (PubMed:25593309). |
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Provided below are standard protocols that you may find useful for product applications.
Background
TIF1 Alpha/TRIM24 is a member of a family of proteins that control transcription and chromatin remodeling through their interaction with transcription factors. The family of TIF1s (transcriptional intermediary factor 1) includes TIF1-alpha, TIF1-beta, and TIF1-gamma. TIF1-alpha plays an important role in development and mediates transcriptional control by interacting with the AF2 region of nuclear receptors such as estrogen, retinoic acid, and vitamin D3 receptors. TIF1-alpha has been shown to be a repressor of transcription. This transcriptional repression involves histone deacetylation and interaction with members of the heterochromatin protein 1 family.
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