|Application ||WB, IP|
|Calculated MW||110417 Da|
|Application & Usage||Western blotting (1:1000 – 1:2500) and Immunoprecipitation. However, the optimal concentrations should be determined individually. HeLa cell lysate can be used as a positive control. The antibody recognizes the CSE1 of human and mouse origins. Reactivity to other species has not been tested.|
|Other Names||CSE1, CSE-1, CSE1L, Chromosome Segregation 1-like, CAS, Cellular Apoptosis Susceptibility Protein, XPO2, XPO-2|
|Formulation||100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||CSE1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Export receptor for importin-alpha. Mediates importin- alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.|
|Cellular Location||Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm|
|Tissue Location||Highly expressed in proliferating cells.|
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Provided below are standard protocols that you may find useful for product applications.
CSE1 function to re-export importin-alpha to the cytoplasm after it has transported nuclear localization signal (NLS) containing proteins to the nucleus. CSE1 is also thought to play a role in cell proliferation and the mitotic spindle checkpoint.
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