|Application ||WB, IP|
|Calculated MW||57862 Da|
|Application & Usage||Western blotting (1:500 – 1:2000) and Immunoprecipitation. However, the optimal concentrations should be determined individually. HeLa cell lysate can be used as a positive control. The antibody recognizes the RCH1 (KPNA2) of human origin. Reactivity to other species has not been tested.|
|Other Names||RCH1, RAG cohort 1, KPNA2, Karyopherin alpha 2, IPOA1, Importin alpha 2, Pendulin, QIP2, SRP1alpha|
|Formulation||100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||RCH1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Expressed ubiquitously.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
The import of proteins from the cytoplasm to the nucleus involves docking the protein to receptors associated with the nuclear pore complex followed by translocation through the nuclear pore. RCH1 is an adaptor protein that recognizes the nuclear localization signal (NLS) on cargo and binds the karyopherin importin-beta receptor for nuclear import which is regulated by RanGTP.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.