|Application ||WB, IP|
|Calculated MW||112135 Da|
|Application & Usage||Western blotting (1:500 – 1:2000) and Immunoprecipitation. However, the optimal concentrations should be determined individually. The antibody recognizes the LOK of human and mouse origins. Reactivity to other species has not been tested.|
|Other Names||LOK, Lymphocyte Oriented Kinase, STK10, STK-10, Serine/Threonine Kinase 10|
|Formulation||100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||LOK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine-protein kinase involved in regulation of lymphocyte migration. Phosphorylates MSN, and possibly PLK1. Involved in regulation of lymphocyte migration by mediating phosphorylation of ERM proteins such as MSN. Acts as a negative regulator of MAP3K1/MEKK1. May also act as a cell cycle regulator by acting as a polo kinase kinase: mediates phosphorylation of PLK1 in vitro; however such data require additional evidences in vivo.|
|Cellular Location||Cell membrane; Peripheral membrane protein|
|Tissue Location||Highly expressed in rapidly proliferating tissues (spleen, placenta, and peripheral blood leukocytes). Also expressed in brain, heart, skeletal muscle, colon, thymus, kidney, liver, small intestine and lung.|
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Provided below are standard protocols that you may find useful for product applications.
The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. LOK (Lymphocyte-oriented kinase), also known as STK10 (serine/threonine kinase 10), is a 968 amino acid protein that contains one protein kinase domain and belongs to the Ser/Thr protein kinase family. Expressed in lymphoid organs, LOK functions to catalyze the ATP-dependent phosphorylation of target proteins, such as MBP (myelin basic protein) and Histone H2A, thereby playing a role in signaling pathways throughout the cell.
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