|Calculated MW||41516 Da|
|Application & Usage||Western blotting (1:500 – 1:2500). However, the optimal concentrations should be determined individually. HeLa cell lysate can be used as a positive control. The antibody recognizes the PRMT1 of human and mouse origins. Reactivity to other species has not been tested.|
|Other Names||PRMT1, PRMT-1, Protein Arginine N-Methyltransferase 1, HRMT1L2, HMT1 hnRNP Methyltransferase-like 2, ANM1, Arginine N-Methyltransferase 1, IR1B4, Interferon Receptor 1-Bound Protein 4, HCP|
|Formulation||100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||PRMT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||HMT2, HRMT1L2, IR1B4|
|Function||Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates FOXO1 and retains it in the nucleus increasing its transcriptional activity. Methylates CHTOP and this methylation is critical for its 5-hydroxymethylcytosine (5hmC)-binding activity (PubMed:25284789). Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner (PubMed:25284789).|
|Cellular Location||Nucleus. Nucleus, nucleoplasm. Cytoplasm, cytosol. Note=Mostly found in the cytoplasm. Colocalizes with CHTOP within the nucleus. Low levels detected also in the chromatin fraction (By similarity).|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
PRMT1 methylate’s (mono & asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in a glycine and arginine-rich domain (may methylate HNRNPA1 and histones) methylate’s SUPT5H. The PRMT1 protein functions as a histone methyltransferase specific for H4. PRMT1 is an essential factor in oncogenesis and is a potential novel therapeutic target in cancer. PRMT1-mediated methylation serves as a positive modulator of IR/IRS-1/PI3K pathway and glucose uptake in skeletal muscle cells. CAF1 is a new regulator of PRMT1-dependent arginine methylation. PRMT1 arginine-methylate’s MRE11 therefore it regulates the activity of MRE11-RAD50-NBS1 complex during the intra-S-phase DNA damage checkpoint response. PRMT1 plays a post-translationally part in regulating the transcriptional activity. PRMT1 is found predominantly in the cytoplasm, though a fraction of PRMT1 is located in the nucleus.
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