|Calculated MW||47535 Da|
|Application & Usage||Western blotting (1:500 – 1:2000). However, the optimal concentrations should be determined individually. HeLa nuclear extract can be used as a positive control. The antibody recognizes the CTBP1 of human and mouse origins. Reactivity to other species has not been tested.|
|Other Names||CTBP, CTBP1, CTBP-1, C-Terminal Binding Protein 1|
|Formulation||100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||CTBP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||Expressed in germinal center B-cells.|
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CtBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CtBP1 and the related protein CtBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CtBP1 is expressed from embryo to adult, but CtBP2 is mainly expressed during embryogenesis. During skeletal and T-cell development, CtBP1 and CtBP2 associate with the PLDLSL domain of ∂EF1, a cellular zinc finger-homeodomain protein, and thereby enhances ∂EF1 induced transcriptional silencing. In addition, CtBP complexes with CtIP, a protein that recognizes distinctly different protein motifs from CtBP. CtIP binds to the BRCT repeats within the breast cancer gene BRCA1 and enables CtBP to influence BRCA1 activity. CtIP/CtBP binding to BRCA1 inhibits the transactivation of the p21 promoter, and it is critical for regulating p21 transcription in response to DNA damage.
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