Prohibitin Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IF, IP |
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Primary Accession | P35232 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 29804 Da |
Gene ID | 5245 |
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Application & Usage | Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml) and Immunofluorescence (5-10 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 33 kDa protein, corresponding to the apparent molecular mass of Prohibitin on SDS-PAGE immunoblots, in samples from human, mouse and rat origins. |
Other Names | PHB, phb |
Target/Specificity | Prohibitin |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Prohibitin Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | PHB1 {ECO:0000303|PubMed:28017329, ECO:0000312|HGNC:HGNC:8912} |
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Function | Protein with pleiotropic attributes mediated in a cell- compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors in the nucleus (PubMed:11302691, PubMed:20959514, PubMed:28017329, PubMed:31522117). Plays a role in adipose tissue and glucose homeostasis in a sex-specific manner (By similarity). Contributes to pulmonary vascular remodeling by accelerating proliferation of pulmonary arterial smooth muscle cells (By similarity). |
Cellular Location | Mitochondrion inner membrane. Nucleus. Cytoplasm. Cell membrane |
Tissue Location | Widely expressed in different tissues. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Prohibitin is an evolutionarily conserved protein that has anti-proliferative activity. Prohibitin is a 30 kDa, post-synthetically modified protein found in the inner membrane of mitochondria and on the plasma membrane of B cells. It mediates B cell mutation. Prohibitin Controls cell cycle in both transcriptional and post-translational mechanisms. Prohibitin is a potential tumor suppressor. Prohibitin gene mutations are correlated with the breast cancer development and/or progression in over 80%0f the cell lines analyzed.
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