|Calculated MW||57223 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 100-120 kDa band, corresponding to glycosylated HSF 1 in samples from human and mouse origins. Reactivity to other species has not been tested.|
|Other Names||HSF-1, HSF 1, Heat shock factor 1, Heat shock transcription factor 1 , HSTF1, HSTF 1|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||HSF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.|
|Cellular Location||Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules (By similarity)|
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Provided below are standard protocols that you may find useful for product applications.
HSF 1 responds to cellular stress signals such as heat, heavy metals and oxidative reagents. It binds to heat shock response elements in the upstream region of heat shock gene and activates transcription. Upon activation, HSF 1 is trimerized and is localized in the nucleus where it binds the DNA.
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