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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   HSF1 Antibody   

HSF1 Antibody

Rabbit Polyclonal Antibody

  • WB - HSF1 Antibody ABV10626
    Western blot analysis of HSF-1 expression in lysates from 3T3 cells (Lane 1) and Jurkat cells (Lane 2,3).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P38532
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 57223 Da
Additional Information
Gene ID 15499
Application & Usage Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 100-120 kDa band, corresponding to glycosylated HSF 1 in samples from human and mouse origins. Reactivity to other species has not been tested.
Other Names HSF-1, HSF 1, Heat shock factor 1, Heat shock transcription factor 1 , HSTF1, HSTF 1
Target/Specificity HSF
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsHSF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Hsf1
Function DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules (By similarity)
Research Areas
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HSF 1 responds to cellular stress signals such as heat, heavy metals and oxidative reagents. It binds to heat shock response elements in the upstream region of heat shock gene and activates transcription. Upon activation, HSF 1 is trimerized and is localized in the nucleus where it binds the DNA.

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$ 245.00
Cat# ABV10626
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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