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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ATGLAntibody   


Rabbit Polyclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96AD5
Other Accession AAW81962
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 55316 Da
Additional Information
Gene ID 57104
Positive Control Western Blot: rat kidney tissue lysate. IHC: Heart tissue
Application & Usage Western blotting (0.5-4 µg/ml), Immunohistochemistry (5 µg/ml), and immunoprecipitation. recognizes 54 kDa ATGL in samples from human, mouse and rat origins. However, the optimal concentrations should be determined individually.
Other Names Adipose Triglyceride Lipase, Patatin-like phospholipase domain-containing protein 2, Desnutrin, Transport-secretion protein 2, TTS2
Target/Specificity ATGL
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsATGLAntibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms ATGL
Function Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes. May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.
Cellular Location Lipid droplet. Cell membrane; Single-pass type II membrane protein
Tissue Location Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level)
Research Areas
Citations (0)

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Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues. Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific. ATGL was independently identified as desnutrin and the TG-hydrolace inducible phospholipase-A2-z.

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$ 245.00
Cat# ABV10628
(40 western blots)
Availability: 5-7days
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