|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||55316 Da|
|Positive Control||Western Blot: rat kidney tissue lysate. IHC: Heart tissue|
|Application & Usage||Western blotting (0.5-4 µg/ml), Immunohistochemistry (5 µg/ml), and immunoprecipitation. recognizes 54 kDa ATGL in samples from human, mouse and rat origins. However, the optimal concentrations should be determined individually.|
|Other Names||Adipose Triglyceride Lipase, Patatin-like phospholipase domain-containing protein 2, Desnutrin, Transport-secretion protein 2, TTS2|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||ATGLAntibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed:15550674). Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes (PubMed:16239926). May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion.|
|Cellular Location||Lipid droplet. Cell membrane; Single-pass type II membrane protein|
|Tissue Location||Highest expression in adipose tissue. Also detected in heart, skeletal muscle, and portions of the gastrointestinal tract. Detected in normal retina and retinoblastoma cells. Detected in retinal pigment epithelium and, at lower intensity, in the inner segments of photoreceptors and in the ganglion cell layer of the neural retina (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
Triglycerides form an important energy store in many living organisms. Adipose tissue serves as the primary storage depot for triglycerides in mammals. Lipolytic enzymes mobilize triglycerides during periods of starvation to provide organisms with necessary energy. Hormone-sensitive lipase (HSL), the first identified lipolytic enzyme, hydrolyzes triglycerides in mammalian adipose tissues. Additional lipolytic enzymes, including adipose triglyceride lipase (ATGL), have also been discovered. The primary function of ATGL is to catalyze the hydrolysis of the first ester bond of lipid molecules. This enzyme may provide diglyceride substrates for HSL hydrolysis. ATGL is abundantly expressed in murine white and brown adipose tissue, and is highly substrate specific. ATGL was independently identified as desnutrin and the TG-hydrolace inducible phospholipase-A2-z.
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