|Calculated MW||61002 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~ 83 kDa Nectin-3 of mouse and rat origins. Reactivity to other species has not been tested.|
|Other Names||Nectin3, Nectin 3, Poliovirus receptor-related protein 3, PVRL3, PVRL-3, PVRL 3, CDw113, CD113, PRR3, PRR-3|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Nectin-3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Nectin-3/CD113 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis- mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.|
|Cellular Location||Cell membrane; Single-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane|
|Tissue Location||Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines|
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Provided below are standard protocols that you may find useful for product applications.
Nectin is a cell-adhesion molecule that belongs to the immunoglobilin superfamily and is also known as the poliovirus receptor-related protein or PPR. Nectin family consists of Nectin 1 which expressed abundantly in the brain and Nectin 2 and 3 which are ubiquitously expressed in cells where cadherin are not expressed. Nectin-3 or PPR3 is a transmembrane protein that has three splicing variants: Nectin3α, Nectin 3β and Nectin 3g. Nectin-3 is mainly expressed in the testis and placental tissues and interacts in vivo with both long and short isoforms of afadin.
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