|Reactivity||Human, Mouse, Rat|
|Calculated MW||85516 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined.|
|Other Names||ASAH-2 , HNAC1 , MGC129777|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-ASAH-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||ASAH2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract.|
|Cellular Location||Cell membrane; Single-pass type II membrane protein. Note=The neutral ceramidase soluble form is a secreted protein. According to PubMed:10781606, it is mitochondrial. However, they used a shorter form in its N- terminus, which may explain this localization which probably does not exist in vivo|
|Tissue Location||Primarily expressed in the intestine (PubMed:17334805). Ubiquitously expressed with higher levels in kidney, skeletal muscle and heart (PubMed:10781606). According to PubMed:17334805, ubiquitous expression attributed to ASAH2 may be actually that of the paralog ASAH2B.|
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Provided below are standard protocols that you may find useful for product applications.
ASAH2 is a key regulator of sphingolipid signaling metabolites. It catalyzes the degradation of sphingolipid C-16 Ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5.
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