|Reactivity||Human, Mouse, Rat|
|Calculated MW||56790 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~60 kDa DGAT-1 of human, mouse, and rat origins. Reactivity to other species has not been tested. |
|Other Names||ARGP1 , DGAT, DGAT1|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-DGAT-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||DGAT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Diacylglycerol acyltransferase is also known as DGAT1 is a microsomal enzyme. It is involved in the synthesis of fatty acids into triglycerides. DGAT utilizes diacylglycerol (DAG) and fatty acyl CoA as substrates to synthesize triacylglycerol. DGAT plays an important role in the metabolism of cellular diacylglycerol and is essential in the physiologic processes of higher eukaryotes that includes triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation.
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