|Reactivity||Human, Mouse, Rat, Sheep, Bovine|
|Calculated MW||86772 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~95-110 kDa Furin/PACE. A splice variant (~60 kDa) can also be detected.|
|Other Names||FES upstream region , FUR , FURIN , PACE , PCSK3 , SPC1|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Furin/PACE polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Furin/PACE Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.|
|Cellular Location||Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Note=Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin (By similarity)|
|Tissue Location||Seems to be expressed ubiquitously.|
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Provided below are standard protocols that you may find useful for product applications.
Furin is a ubiquitous protein located within the trans-Golgi network. It is found in many tissues and cell lines including MDCK, HeLa, HepG2 and NIH-3T3. Furin is a proprotein convertase that is responsible for the proteolytic maturation of many precursor proteins that are secreted from the constitutive secretory pathway
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