|Calculated MW||147182 Da|
|Application & Usage||Western blotting (1:200 – 1:2000). However, the optimal concentrations should be determined individually. HEK293T cell lysate can be used as a positive control. The antibody recognizes the SMC4 of human origin. Reactivity to other species has not been tested.|
|Other Names||CAPC , SMC4 , SMC4L1 , hCAP-C|
|Formulation||100 µL affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||SMC4/CAPC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.|
|Cellular Location||Nucleus. Cytoplasm. Chromosome. Note=In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase|
|Tissue Location||Widely expressed. Higher expression in testis, colon, thymus.|
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Provided below are standard protocols that you may find useful for product applications.
The SMC (structural maintenance of chromosomes) family of proteins form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation for mitosis. The two distinct classes of SMC protein complexes are comprised of SMC1 (also designated SB1.8) with SMC3 (also designated hCAP and Bamacan), and SMC2 (also designated hCAP-E for chromosome-associated polypeptide E) with hCAP-C. The SMC2/hCAP-C complex is required for mitotic chromosome condensation and functions independently of the SMC1/SMC3 complex during the cell cycle. Both SMC2 and hCAP-C are expressed throughout the cell cycle in a variety of cell lines, including HeLa cells, Daudi B cells, Jurkat T cells, SK2 neuronal cells, and HepG2 liver cells.
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