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Sphingosine Kinase 1 (SPK1) Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q8CI15 |
| Other Accession | CAM17115 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 42443 Da |
| Gene ID | 20698 |
|---|---|
| Application & Usage | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 42 kDa bands from samples of human, mouse and rat origins. Reactivity to other species has not been tested. |
| Other Names | SPK1, SPK-1, SPK 1, sphk, SK1, SK 1 |
| Target/Specificity | SPK1 |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.5mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | Sphingosine Kinase 1 (SPK1) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Sphk1 {ECO:0000312|MGI:MGI:1316649} |
|---|---|
| Function | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions (PubMed:17346996, PubMed:21084291, PubMed:25417698, PubMed:29662056, PubMed:33334894). Also acts on D- erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol (PubMed:9726979). In contrast to proapoptotic SPHK2, has a negative effect on intracellular ceramide levels, enhances cell growth and inhibits apoptosis (PubMed:16118219). Involved in the regulation of inflammatory response and neuroinflammation. Via the product sphingosine 1-phosphate, stimulates TRAF2 E3 ubiquitin ligase activity, and promotes activation of NF-kappa-B in response to TNF signaling (By similarity). In response to TNF and in parallel to NF-kappa-B activation, negatively regulates RANTES induction through p38 MAPK signaling pathway (By similarity). Involved in endocytic membrane trafficking induced by sphingosine, recruited to dilate endosomes, also plays a role on later stages of endosomal maturation and membrane fusion independently of its kinase activity (PubMed:27806293, PubMed:28049734). In Purkinje cells, seems to be also involved in the regulation of autophagosome-lysosome fusion upon VEGFA (PubMed:25417698). |
| Cellular Location | Cytoplasm. Endosome membrane; Peripheral membrane protein. Nucleus. Cell membrane {ECO:0000250|UniProtKB:Q9NYA1}. Synapse Note=Translocated from the cytoplasm to the plasma membrane in a CIB1- dependent manner. Binds to membranes containing negatively charged lipids but not neutral lipids (By similarity). Recruited to endocytic membranes by sphingosine where promotes membrane fusion (PubMed:27806293). {ECO:0000250|UniProtKB:Q9NYA1, ECO:0000269|PubMed:27806293} |
| Tissue Location | Widely expressed (PubMed:9726979). Expressed in brain (at protein level). Detected in neurons |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Sphingosine Kinase (SphK) is a conserved lipid kinase that catalyzes the phosphorylation of sphingolipid sphingosine to sphingosine-1-phosphate (SIP). SIP receptors coupled to cell surface G protein to regulate cell growth, survival, motility and inflammatory responses. There are two types of SphK, SphK1 and SphK2. SphK1 is found in the cytosol and migrates to the membrane when activated. SphK1 has been associated with cell growth, prevention of apoptosis and cellular transformation. SphK2 is found mainly in the nucleus and in contrast to SphK1, it enhances the rate of apoptosis.
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