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TFAM Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q91ZW1 |
| Reactivity | Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 28187 Da |
| Gene ID | 83474 |
|---|---|
| Application & Usage | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 27 kDa band from rat kidney tissue lysate. Reactivity to other species has not been tested. |
| Other Names | Transcription factor A, MtTF1, TCF6, TCF6L2, mtTFA |
| Target/Specificity | TFAM |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.5mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | TFAM Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Tfam {ECO:0000312|RGD:620682} |
|---|---|
| Function | [Isoform Mitochondrial]: Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA (By similarity). |
| Cellular Location | [Isoform Mitochondrial]: Mitochondrion {ECO:0000250|UniProtKB:Q00059}. Mitochondrion matrix, mitochondrion nucleoid {ECO:0000250|UniProtKB:Q00059} |
| Tissue Location | The mitochondrial isoform is widely expressed while the nuclear isoform is testis-specific |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Transcription factor A mitochondria (TFAM) is an important activator of mitochondrial DNA transcription and replication. Studies in mice have shown that TFAM is required to regulate the mitochondrial genome copy number and is important for embryonic development. TFAM has been associated with mitochondrial disorder which includes ocular myopathy and muscular wasting in humans. Kearns-Sayre syndrome was shown in mouse model when expression of the gene was eliminated by targeted disruption in heart and muscle cells.
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