|Calculated MW||28187 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 27 kDa band from rat kidney tissue lysate. Reactivity to other species has not been tested. |
|Other Names||Transcription factor A, MtTF1, TCF6, TCF6L2, mtTFA|
|Formulation||100 µg (0.5mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||TFAM Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Isoform Mitochondrial binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA (By similarity). Isoform Nuclear may also function as a transcriptional activator or may have a structural role in the compaction of nuclear DNA during spermatogenesis (By similarity).|
|Cellular Location||Isoform Mitochondrial: Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid|
|Tissue Location||The mitochondrial isoform is widely expressed while the nuclear isoform is testis-specific|
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Provided below are standard protocols that you may find useful for product applications.
Transcription factor A mitochondria (TFAM) is an important activator of mitochondrial DNA transcription and replication. Studies in mice have shown that TFAM is required to regulate the mitochondrial genome copy number and is important for embryonic development. TFAM has been associated with mitochondrial disorder which includes ocular myopathy and muscular wasting in humans. Kearns-Sayre syndrome was shown in mouse model when expression of the gene was eliminated by targeted disruption in heart and muscle cells.
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