|Reactivity||Human, Mouse, Rat, Pig, Bovine|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~37 kDa Apg5/Atg5 from samples of human, mouse and rat origins. Reactivity to other species has not been tested.|
|Other Names||Autophagy protein 5, APG5-like , Apoptosis-specific protein, autophagy protein, atg5, atg-5, apg5, apg-5|
|Formulation||100 µg (0.5mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Apg5/Atg5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Autophagy, the process of bulk degradation of cellular proteins thro µgh an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. An ubiquitin like conj µgation system is involve in the formation of autophagosome where by Atg12 is covanletly bound to Apg5/ATG5. The conj µgation is mediated by the ubiquitin-E1-like enzyme Atg7 and thE2-like enzyme Atg10.
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