|Reactivity||Human, Mouse, Rat|
|Calculated MW||81925 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been tested. The antibody detects ~86-100 kDa Sp3 in samples from human, mouse and rat origins.|
|Other Names||Sp-3, Sp3, SP 3, SP-3, SP3 transcription factor, transcription factor SP 3, Transcription factor SP3|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Sp3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Sp3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone- induction and house-keeping.|
|Cellular Location||Nucleus. Nucleus, PML body. Note=Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies|
|Tissue Location||Ubiquitously expressed.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Sp transcription factor family has a highly conserved DNA-binding domain composed of zinc fingers. The Sp family is comprised of Sp1, Sp2, Sp3 and Sp4 that regulates transcription by binding to consensus GC- and GT-box regulatory elements in the target genes. Sp1, Sp3 and Sp4 have a high binding capacity for GC box while Sp2 binds weakly to the GT box. Sp3 has been shown to have to a bifunctional transcription factor that can either stimulates or represses the transcription of various genes.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.