|Reactivity||Human, Mouse, Rat|
|Calculated MW||81925 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been tested. The antibody detects ~86-100 kDa Sp3 in samples from human, mouse and rat origins.|
|Other Names||Sp-3, Sp3, SP 3, SP-3, SP3 transcription factor, transcription factor SP 3, Transcription factor SP3|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Sp3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Sp3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone- induction and house-keeping.|
|Cellular Location||Nucleus. Nucleus, PML body. Note=Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies|
|Tissue Location||Ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Sp transcription factor family has a highly conserved DNA-binding domain composed of zinc fingers. The Sp family is comprised of Sp1, Sp2, Sp3 and Sp4 that regulates transcription by binding to consensus GC- and GT-box regulatory elements in the target genes. Sp1, Sp3 and Sp4 have a high binding capacity for GC box while Sp2 binds weakly to the GT box. Sp3 has been shown to have to a bifunctional transcription factor that can either stimulates or represses the transcription of various genes.
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