|Reactivity||Human, Mouse, Rat|
|Calculated MW||24261 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~40 kDa of Dkk4 in samples from human, mouse and rat origins. Reactivity to other species has not been determined.|
|Other Names||DKK-4, DKK 4, dkk4, dkk-4, dkk 4, dickkopf homolog-4, dickkopf homolog4, dickkopf homolog 4|
|Formulation||100 µg (0.5 mg/ml) antigen affinity purified rabbit anti-Dkk4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol and 0.01% Thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||DKK4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
Xenopus Dickkopf (Dkk) was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of Dkk have been identified in mammals. Each Dkk molecule contains two conserved cysteine-rich domains which are separated by a 50-55 amino acid linker region. Dkk4 is a secreted glycoprotein and has three immune-reactive sites at 15-17kDa, 30-32kDa and at 40kDa.
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