|Reactivity||Human, Mouse, Rat|
|Calculated MW||73701 Da|
|Positive Control||Rat Kidney tissue lysate|
|Application & Usage||Western blot: (1:200 dilution) Use 0.5 – 4 µg/ml based on your sample type.|
|Other Names||ER degradation-enhancing alpha-mannosidase-like protein 1|
|Formulation||100 µg (0.5 mg/ml) antibody in 1 x PBS pH 7.2, 0.01 % BSA, 0.01 % thimerosal, and 50 % glycerol.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||EDEM1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type II membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
EDEM1 belongs to the glycosyl hydrolase 47 family. It extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-dependent manner.
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