|Reactivity||Human, Mouse, Rat, Dog|
|Calculated MW||80745 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~80 kDa of Pak5/PAK7 from samples of human, mouse, rat and dog origins. Reactivity to other species has not been determined. |
|Other Names||Serine/threonine-protein kinase PAK 7; p21-activated kinase 7; PAK-7; p21-activated kinase 5; PAK-5.|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-human PAK5/PAK7 polyclonal antibody in phosphate (PBS, pH 7.2) containing 30% glycerol, 0.5 % BSA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||PAK5/PAK7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.|
|Cellular Location||Mitochondrion. Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the mitochondria, and mitochondrial localization is essential for the role in cell survival|
|Tissue Location||Predominantly expressed in brain.|
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Provided below are standard protocols that you may find useful for product applications.
The PAK (p21-activated kinase) family of serine/threonine kinases plays an important role in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, etc. PAK 5 is known for promoting neurite outgrowth and thus predominantly expressed in the brain. PAK 5 contains CDC42/Rac1and has shown to bind CDC42 in the presence of GTP. PAK 5 also plays an important role in inhibiting apoptotic cascade thro µgh BAD phosphorylation.
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