|Reactivity||Human, Mouse, Rat, Bovine, Dog, Cat|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~34 kDa of Bmi-1 in Jurkat cell lysate and rat kidney tissue lysate. Reactivity to other species has not been tested. |
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||BMI-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Polycomb group (PcG) genes encode chromatin proteins that are involved in the maintenance of cellular memory thro µgh epigenetic chromatin modifications. Bmi-1 has been identified among the PcG genes as a potent oncogene that plays an important role in the regulation of cell proliferation by suppressing the p16-dependant pathway. Bmi-1 may be use as a therapeutic target for studying stem cell proliferation and renewal.
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