|Reactivity||Human, Mouse, Rat|
|Calculated MW||90588 Da|
|Positive Control||Jurkat cell lysate|
|Application & Usage||Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate can be used as a positive control.|
|Other Names||peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PPAR Gamma Coactivator-1, Pgc-1alphaa, PPAR gamma coactivator variant form, PGC-1-alpha, PPARGC-1-alpha, PPAR-gamma coactivator 1-alpha|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-PGC-1alpha polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||PGC-1alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||Pgc1, Pgc1a, Ppargc1|
|Function||Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK. Isoform 4 specifically activates the expression of IGF1 and suppresses myostatin expression in skeletal muscle leading to muscle fiber hypertrophy.|
|Cellular Location||Nucleus. Nucleus, PML body.|
|Tissue Location||White quadriceps and red tibialis anterior (TA) muscles, liver, kidney and brown adipose tissue (at protein level). Skeletal muscle, brown adipose tissue, heart, kidney and brain.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may also be involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.
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