|Reactivity||Human, Mouse, Rat|
|Calculated MW||59978 Da|
|Positive Control||Jurkat cell lysate, 3T3 cell lysate|
|Application & Usage||Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. Jurkat or 3T3 cell lysate can be used as a positive control.|
|Other Names||pyruvate dehydrogenase (lipoamide) E1 component, beta subunit precursor; pyruvate decarboxylase.; pyruvate dehydrogenase, Pyruvic dehydrogenase, Sphingobacterium spiritivorum ATCC 33861|
|Formulation||200 µg (0.5 mg/ml) affinity purified rabbit anti-PDPC polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Pyruvate Dehydrogenase Phosphatase 2 (PDP2) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex.|
|Cellular Location||Mitochondrion matrix.|
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Provided below are standard protocols that you may find useful for product applications.
PDP2, Pyruvate dehydrogenase phosphatase 2, catalyzes the dephosphorylation and activation of E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex.
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