|Reactivity||Human, Mouse, Rat|
|Calculated MW||53082 Da|
|Positive Control||3T3 cell lysate, rat kidney tissue lysate|
|Application & Usage||Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Rat kidney tissue lysate or 3T3 cell lysate can be used as a positive control.|
|Other Names||prelipoprotein lipase, lipo_lipase, lipoprotein lipase,|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-LPL polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||LPL Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium (By similarity).|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Note=Locates to the plasma membrane of microvilli of hepatocytes with triacyl- glycerol-rich lipoproteins (TRL). Some of the bound LPL is then internalized and located inside non-coated endocytic vesicles (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
Lipoprotein lipase (LPL) is mainly expressed in the heart, muscle and adipose tissue. LPL is a homodimer that mediates the hydrolysis of triglycerides of very low density lipoproteins and also function as ligand/bridging factor for receptor-mediated lipoprotein uptake. Defects in LPL may result in hypertriglceridemia.
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