|Reactivity||Human, Mouse, Rat|
|Calculated MW||191570 Da|
|Positive Control||Jurkat, HeLa, 3T3 cell lysates|
|Application & Usage||The antibody can be used for Western blotting (1–4 µg/ml).|
|Other Names||RICTOR; DKFZp686B11164; KIAA1999; MGC39830; mAVO3|
|Formulation||200 µg (0.5 mg/ml) affinity purified rabbit anti-Rictor polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Rictor Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.|
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Provided below are standard protocols that you may find useful for product applications.
Rictor is a component of a protein complex [mTORC2] that integrates nutrient- and growth factor-derived signals to regulate cell growth. Rictor also plays an essential role in Akt phosphorylation and signaling.
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