|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||124412 Da|
|Positive Control||Western Blot: Jurkat cell lysate. IHC: colon tissue|
|Application & Usage||The antibody can be used for Western blot analysis (1-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately.|
|Other Names||PIK3CA; MGC142161; MGC142163; PI3K|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-P13 kinase polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||PI3 kinase Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1- AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS.|
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Provided below are standard protocols that you may find useful for product applications.
Phosphatidylinositol 3-kinase (PI 3-kinase), is a phosphoinositide-specific protein kinase that plays a central role in mitogenic signal transduction leading to cellular regulation, cell growth and differentiation, and oncogenesis. PI 3-kinase associates with, and is activated by a wide range of tyrosine kinase receptors including EGF, PDGF, CSF-1, erbB-3 and insulin receptor, non-receptor protein-tyrosine kinases of the src family (e.g. pp60src), and crk and abl proto-oncogene products. PI 3-kinase is involved in signal transduction in terminally differentiated, activated cells like platelets and neutrophils.
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