|Reactivity||Human, Mouse, Rat|
|Positive Control||Jurkat, Rat kidney, 3T3 cell lysates|
|Application & Usage||The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately.|
|Other Names||CBP-tag, Calmodulin binding peptide-tag|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-CBP-Tag polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||CBP-Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Calmodulin binding peptide (CBP) tag is a fusion protein that is useful for immunoaffinity purification of proteins expressed in E.coli. The CBP tag binds to calmodulin resin in the presence of low concentration of calcium and can be eluted with a neutral buffer. The antibody is suitable for identifying CBP-fusion proteins.
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