Sin1 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB |
---|---|
Primary Accession | Q8BKH7 |
Other Accession | NP_796319 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 59009 Da |
Gene ID | 227743 |
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Positive Control | Jurkat (Induced and Uninduced), Rat kidney, 3T3 cell lysates |
Application & Usage | The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately. |
Other Names | MAPKAP1; MIP1; MGC2745; SIN1; SIN1b; SIN1g |
Target/Specificity | Sin1 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml) affinity purified rabbit anti-Sin1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Sin1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Mapkap1 |
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Synonyms | Mip1, Sin1 |
Function | Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Isoform 1 is involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex. |
Cellular Location | Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle. Nucleus |
Tissue Location | Uniquitously expressed, with highest levels in testis, kidney and liver. Present in renal tubule cells (at protein level). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Stress-activated map kinase-interacting protein 1 or Sin1 is required for Rictor binding in the mTORC2 complex. Sin1 is also a scaffolding protein in SAPK (stress-activated kinase) signaling and JNK signaling pathway.
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