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Sin1 Antibody

Rabbit Polyclonal Antibody

  • WB - Sin1 Antibody ABV10752
    Western blot analysis of Sin1 with Jurkat cell lysates (Lane 1) and rat kidney tissue lysate (Lane 2).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8BKH7
Other Accession NP_796319
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 59009 Da
Additional Information
Gene ID 227743
Positive Control Jurkat (Induced and Uninduced), Rat kidney, 3T3 cell lysates
Application & Usage The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately.
Other Names MAPKAP1; MIP1; MGC2745; SIN1; SIN1b; SIN1g
Target/Specificity Sin1
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit anti-Sin1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsSin1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Mapkap1
Synonyms Mip1, Sin1
Function Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Isoform 1 is involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.
Cellular Location Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle Nucleus
Tissue Location Uniquitously expresseed, with highest levels in testis, kidney and liver. Present in renal tubule cells (at protein level).
Research Areas
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Stress-activated map kinase-interacting protein 1 or Sin1 is required for Rictor binding in the mTORC2 complex. Sin1 is also a scaffolding protein in SAPK (stress-activated kinase) signaling and JNK signaling pathway.

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$ 245.00
Cat# ABV10752
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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