|Reactivity||Human, Mouse, Rat|
|Calculated MW||59009 Da|
|Positive Control||Jurkat (Induced and Uninduced), Rat kidney, 3T3 cell lysates|
|Application & Usage||The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately.|
|Other Names||MAPKAP1; MIP1; MGC2745; SIN1; SIN1b; SIN1g|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Sin1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Sin1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient- insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Isoform 1 is involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.|
|Cellular Location||Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle Nucleus|
|Tissue Location||Uniquitously expresseed, with highest levels in testis, kidney and liver. Present in renal tubule cells (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
Stress-activated map kinase-interacting protein 1 or Sin1 is required for Rictor binding in the mTORC2 complex. Sin1 is also a scaffolding protein in SAPK (stress-activated kinase) signaling and JNK signaling pathway.
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