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Strep-Tag II Antibody

Rabbit Polyclonal Antibody

     
  • WB - Strep-Tag II Antibody ABV10757
    Western blot analysis of Strep-Tag II protein ladder (Lane 1) and ApoE4 control protein without Strep-Tag II(Lane 2) using anti-Strep-Tag II antibody.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Reactivity All Species
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Additional Information
Positive Control Strep-Tag II protein ladder (Lane 1)
Application & Usage The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually.
Other Names Strep-Tag II, anti-Strep-Tag II, Strep-Tag II antibody, Strep-Tag IIantibody
Target/Specificity Strep-Tag II
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit anti-Strep-Tag II polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 5 mM EDTA and 0.01 % thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsStrep-Tag II Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)

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Background

Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.

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$ 89.00
$ 265.00
Cat# ABV10757
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Availability: 5-7days
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