|Positive Control||Strep-Tag II protein ladder (Lane 1)|
|Application & Usage||The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||Strep-Tag II, anti-Strep-Tag II, Strep-Tag II antibody, Strep-Tag IIantibody|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Strep-Tag II polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 5 mM EDTA and 0.01 % thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Strep-Tag II Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
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