|Application & Usage||Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml) and immunofluorescence. However, the optimal conditions should be determined individually.|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||YFP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. YFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or YFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or YFP can fuse with proteins of interest without interfering significantly with their assembly and function.
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