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Green Fluorescence Protein (GFP) Antibody (Clone BV-F4)

Mouse Monoclonal Antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Other Accession ABG78037
Reactivity All Species
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names BV-F4
Additional Information
Application & Usage Western blotting (0.5-4 µg/ml), immunoprecipitation (2-8 µg/ml), immunofluorescence (10-20 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes wild-type GFP and its derivatives such as EGFP, EBFP, ECFP, and EYFP, etc. Performs well in Western blot analysis (0.5 to 4 µg/ml), immunoprecipitation, and Immunohistochemistry. However, the optimum conditions should be determined individually.
Target/Specificity GFP
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) antigen affinity purified mouse anti-GFP monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsGreen Fluorescence Protein (GFP) Antibody (Clone BV-F4) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)
citation

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Background

Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can also fuse with proteins of interest without interfering significantly with their assembly and function.

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Discontinued
Cat# ABV10760-100
Size:

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