|Application & Usage||Western blotting (0.5-4 µg/ml), immunoprecipitation (2-8 µg/ml), immunofluorescence (10-20 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes wild-type GFP and its derivatives such as EGFP, EBFP, ECFP, and EYFP, etc. Performs well in Western blot analysis (0.5 to 4 µg/ml), immunoprecipitation, and Immunohistochemistry. However, the optimum conditions should be determined individually.|
|Formulation||100 µg (0.5 mg/ml) antigen affinity purified mouse anti-GFP monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Green Fluorescence Protein (GFP) Antibody (Clone BV-F4) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can also fuse with proteins of interest without interfering significantly with their assembly and function.
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