|Calculated MW||3936 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). Based on researchers’ feedback, it can also be used in immunoprecipitation (10-20 µg/ml) and immunofluorescence. However, the optimal conditions should be determined individually.|
|Other Names||Red Fluorescent Protein|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-RFP polyclonall antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||RFP (dsRed) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Nonheme iron protein possibly involved in electron transport.|
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Provided below are standard protocols that you may find useful for product applications.
Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function.
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