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CD40L Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P27548 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 29370 Da |
| Gene ID | 21947 |
|---|---|
| Application & Usage | Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate can be used as a positive control. |
| Other Names | CD40L, CD40 Ligand, CD40Ligand, CD40-L, CD40-Ligand |
| Target/Specificity | CD40L |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.5 mg/ml) Protein A affinity purified rabbit anti-mouse CD40L polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA and 0.01% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | CD40L Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Cd40lg |
|---|---|
| Synonyms | Cd40l, Tnfsf5 |
| Function | Cytokine that acts as a ligand to CD40/TNFRSF5 (By similarity). Costimulates T-cell proliferation and cytokine production (By similarity). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (By similarity). Induces the activation of NF-kappa-B (By similarity). Induces the activation of kinases MAPK8 and PAK2 in T-cells (By similarity). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (PubMed:1374165). Involved in immunoglobulin class switching (PubMed:1374165). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P29965}; Single-pass type II membrane protein {ECO:0000250|UniProtKB:P29965} Cell surface {ECO:0000250|UniProtKB:P29965} |
| Tissue Location | Specifically expressed on activated CD4+ T- lymphocytes |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
CD40, also known as bp50 or CDW40, is a type I membrane protein found on the surface of B cells and primary carcinomas. It belongs to the TNF-R family. CD40 serves as the receptor for CD40 ligand (CD40L), a cytokine expressed on the cell surface of T cells. CD40 plays a critical role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center thro µgh interaction with T cells expressing CD40L, and activation of CD4+ T cells. Ligation of CD40 with its ligand preferentially induces stress-activated protein kinases in B lymphocytes.
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