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EGF Antibody

Rabbit Polyclonal Antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P01133
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 133994 Da
Additional Information
Gene ID 1950
Application & Usage Western blot analysis (0.5-4 µg/ml). Recombinant human EGF can be used as a positive control. Per customer’s feedback, it can also be used in Neutralization and Elisa. However, the optimal conditions should be determined individually.
Other Names Epidermal Growth Factor
Target/Specificity EGF
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit anti-human EGF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol and 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsEGF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EGF
Function EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
Cellular Location Membrane; Single-pass type I membrane protein.
Tissue Location Expressed in kidney, salivary gland, cerebrum and prostate.
Research Areas
Citations (0)
citation

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Background

EGF (Epidermal Growth Factor) is polypeptide growth factor that stimulates the proliferation of a wide range of epidermal and epithelial cells. Human EGF is a 6.2 kDa protein containing 53 amino acid residues.

FeedBack
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If you have any additional inquiries please email technical services at tech@abcepta.com.

Discontinued
Cat# ABV10782-30
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